BurstProbe: Debugging Time-Critical Data Delivery in Wireless Sensor Networks

In this paper we present BurstProbe, a new technique to accurately measure link burstiness in a wireless sensor network employed for time-critical data delivery. Measurement relies on shared probing slots that are embedded in the transmission schedule and used by nodes to assess link burstiness over time. The acquired link burstiness information can be stored in the node's flash memory and relied upon to diagnose transmission problems when missed deadlines occur. Thus, accurate diagnosis is achieved in a distributed manner and without the overhead of transmitting rich measurement data to a central collection point. For the purpose of evaluation we have implemented BurstProbe in the GinMAC WSN protocol and we are able to demonstrate it is an accurate tool to debug time-critical data delivery. In addition, we analyze the cost of implementingBurstProbe and investigate its effectiveness

The role of mental health in multiple exclusion homelessness

Development of effective policy responding to recent increases in rough sleeping in England requires an understanding of the needs of people experiencing homelessness. Research on multiple exclusion homelessness (MEH) should be central to policy development as it highlights the interactions between ‘homelessness’ and ‘deep social exclusion’. This article focuses on the nexus between mental health and homelessness within MEH. Mixed methods findings from Nottingham, England, are used to investigate the role of mental ill-health in the pathways into MEH. This included a survey of the mental health needs of homeless people and ‘homeless pathway’ interviews. Survey results demonstrated high levels of diagnosed and self-reported mental health issues. Interviews highlighted that mental ill-health and homelessness were closely associated with adverse life experiences and could not be viewed in isolation from them. The article contributes further insight on the specificities of mental health issues, their temporal ordering, and interrelationship with homelessness experiences. between mental health and homelessness within MEH. Mixed methods findings from Nottingham, England, are used to investigate the role of mental ill-health in the pathways into MEH. This included a survey of the mental health needs of homeless people and 'homeless pathway' interviews. Survey results demonstrated high levels of diagnosed and self-reported mental health issues. Interviews highlighted that mental ill-health and homelessness were closely associated with adverse life experiences and could not be viewed in isolation from them. The article contributes further insight on the specificities of mental health issu

Second Set of Spaces

This document describes the Gloss infrastructure supporting implementation of location-aware services. The document is in two parts. The first part describes software architecture for the smart space. As described in D8, a local architecture provides a framework for constructing Gloss applications, termed assemblies, that run on individual physical nodes, whereas a global architecture defines an overlay network for linking individual assemblies. The second part outlines the hardware installation for local sensing. This describes the first phase of the installation in Strathclyde University

Decision-making in COVID-19 and Frailty

Peer reviewedPublisher PD

pH Dependent C-jejuni Thermal Inactivation Models And Application To Poultry Scalding

Campylobacter jejuni related outbreaks and prevalence on retail poultry products pose threats to public health and cause financial burden worldwide. To resolve these problems, it is imperative to take a closer look at poultry processing practices and standards. Using available data (D-values) on the thermal inactivation of C. jejuni we develop a comprehensive inactivation model, taking into account the variation of strain-specific heat resistance, experimental method, and suspension pH. Utilizing our C. jejuni thermal inactivation model, we study the poultry scalding process. We present a mechanistic model of bacteria transfer and inactivation during a typical immersion scald in a high-speed industrial plant. Integration of our C. jejuni inactivation model into the scalding model culminates in validation against industrial processing data. In particular, we successfully predict bacteria concentrations in the scald water and link key factors such as scald water pH and temperature to cross-contamination and overall microbiological quality of carcasses. Furthermore, we demonstrate the applicability of our inactivation model for scalding operations at seven Canadian poultry plants. In addition to providing recommendations for best-practice and a review of scalding research, our work is intended to act as a modular foundation for further research in the interest of public health and financial well-being. (C) 2017 Elsevier Ltd. All rights reserved

Coffee cysteine proteinases and related inhibitors with high expression during grain maturation and germination

<p>Abstract</p> <p>Background</p> <p>Cysteine proteinases perform multiple functions in seeds, including participation in remodelling polypeptides and recycling amino acids during maturation and germination. Currently, few details exist concerning these genes and proteins in coffee. Furthermore, there is limited information on the cysteine proteinase inhibitors which influence the activities of these proteinases.</p> <p>Results</p> <p>Two cysteine proteinase (CP) and four cysteine proteinase inhibitor (CPI) gene sequences have been identified in coffee with significant expression during the maturation and germination of coffee grain. Detailed expression analysis of the cysteine proteinase genes CcCP1 and CcCP4 in Robusta using quantitative RT-PCR showed that these transcripts accumulate primarily during grain maturation and germination/post germination. The corresponding proteins were expressed in <it>E. coli </it>and purified, but only one, CcCP4, which has a KDDL/KDEL C-terminal sequence, was found to be active after a short acid treatment. QRT-PCR expression analysis of the four cysteine proteinase inhibitor genes in Robusta showed that CcCPI-1 is primarily expressed in developing and germinating grain and CcCPI-4 is very highly expressed during the late post germination period, as well as in mature, but not immature leaves. Transcripts corresponding to CcCPI-2 and CcCPI-3 were detected in most tissues examined at relatively similar, but generally low levels.</p> <p>Conclusions</p> <p>Several cysteine proteinase and cysteine proteinase inhibitor genes with strong, relatively specific expression during coffee grain maturation and germination are presented. The temporal expression of the CcCP1 gene suggests it is involved in modifying proteins during late grain maturation and germination. The expression pattern of CcCP4, and its close identity with KDEL containing CP proteins, implies this proteinase may play a role in protein and/or cell remodelling during late grain germination, and that it is likely to play a strong role in the programmed cell death associated with post-germination of the coffee grain. Expression analysis of the cysteine proteinase inhibitor genes suggests that CcCPI-1 could primarily be involved in modulating the activity of grain CP activity; while CcCPI-4 may play roles modulating grain CP activity and in the protection of the young coffee seedlings from insects and pathogens. CcCPI-2 and CcCPI-3, having lower and more widespread expression, could be more general "house-keeping" CPI genes.</p

Individual Based Modeling And Analysis Of Pathogen Levels In Poultry Chilling Process

Pathogen control during poultry processing critically depends on more enhanced insight into contamination dynamics. In this study we build an individual based model (IBM) of the chilling process. Quantifying the relationships between typical Canadian processing specifications, water chemistry dynamics and pathogen levels both in the chiller water and on individual carcasses, the IBM is shown to provide a useful tool for risk management as it can inform risk assessment models. We apply the IBM to Campylobacter spp. contamination on broiler carcasses, illustrating how free chlorine (FC) sanitization, organic load in the water, and pre-chill carcass pathogen levels affect pathogen levels of post-chill broilers. In particular, given a uniform distribution of Campylobacter levels on incoming poultry we quantify the efficacy of FC control in not only reducing pathogen levels on average, but also the variation of pathogen levels on poultry exiting the chill tank. Furthermore, we demonstrate that the absence/presence of FC input dramatically influences when, during a continuous chilling operation, cross-contamination will be more likely

Individual Based Modeling And Analysis Of Pathogen Levels In Poultry Chilling Process

Pathogen control during poultry processing critically depends on more enhanced insight into contamination dynamics. In this study we build an individual based model (IBM) of the chilling process. Quantifying the relationships between typical Canadian processing specifications, water chemistry dynamics and pathogen levels both in the chiller water and on individual carcasses, the IBM is shown to provide a useful tool for risk management as it can inform risk assessment models. We apply the IBM to Campylobacter spp. contamination on broiler carcasses, illustrating how free chlorine (FC) sanitization, organic load in the water, and pre-chill carcass pathogen levels affect pathogen levels of post-chill broilers. In particular, given a uniform distribution of Campylobacter levels on incoming poultry we quantify the efficacy of FC control in not only reducing pathogen levels on average, but also the variation of pathogen levels on poultry exiting the chill tank. Furthermore, we demonstrate that the absence/presence of FC input dramatically influences when, during a continuous chilling operation, cross-contamination will be more likely